Sentence examples for mutants was constructed from inspiring English sources

A library of 1410 single mutants was constructed by saturation mutagenesis that targeted 15 amino acid positions of the active site, identified by molecular modelling.

A series of mutants was constructed to inactivate the thiolation (T) domain of each module and to evaluate an HHxxxDR catalytic motif in NocA and an atypical extended histidine motif in NocB.

To evaluate the role of domain I of calmodulin (CaM) in the activation of target enzymes, a series of CaM mutants was constructed in which domain I (49 amino acids) was substantially deleted, or was exchanged with the homologous region (58 amino acids) of cardiac troponin C (cTnC).

A series of APOBEC3G alanine and specific mutants was constructed with the QuickChange Mutagenesis kit (Stratagene).

To further elucidate the domain of RCAN1 interacting with I κB α, a series of deletion mutants was constructed.

To address this issue, a full-length HBx fragment that contains the same point mutations as the deletion mutants was constructed and used as the control.

Overexpression mutants were constructed by using directional pBAD plasmid system (Invitrogen) according to the manufacturer's instructions.

Affinity mutants were constructed with binding constants ranging from 50 μM to 1 mM.

In addition, two more mutants were constructed by splicing of the C-terminal non-conserved sequence.

The reactions are modelled using semi-empirical quantum-mechanic methods and the protein mutants are constructed by homology modeling.

On the basis of the predictions from this algorithm, mutants were constructed and characterized for a model protein, thioredoxin.