Sentence examples for mutants was conducted from inspiring English sources

An expression analysis of the genes in the lov1-4 mutants was conducted to confirm that LOV1 affects the expressions of CO and FT but not GI.

A pilot study of the feeding assay using wild-type and four eat mutants was conducted to determine the number of experimental replicates required for optimal statistical power.

Site-specific mutagenesis to obtain ComFB mutants was conducted using standard protocols using Pfu polymerase and two oligonucleotides for each mutation (oTS387/oTS388; oTS387/oTS388; oTS387/oTS388).

Screening for atocd-2/p5cs1-4 atocd-2/p5cs1-4 atocd-2/p5cs1-4ing primers given in Adoublenal file 1: Table S2 and those reported previously for p5cs1-4[ 14].

To determine if there were any alterations in the overall extractability of non-cellulosic glycan epitopes in the fpgs1- 1 mutant, glycome profiling of the cell wall material (alcohol insoluble residues; AIR) isolated from 35-day-old inflorescence stems of wild-type and fpgs1- 1 mutants was conducted (Fig.  4a).

Protein expression and purification of these full length eNOS or nNOS mutants were conducted using the established procedure reported previously.

The expression and purification of SwH-NOX surface mutants were conducted using the same protocols as described above for the wild-type SwH-NOX protein.

Tranfections of T47D-Y cells with C587A-PR-B and PR-BmPro mutants were conducted as we previously described using vector concentrations which resulted in mutant PRs expression levels comparable to those present in T47D cells [ 5].

Microarray comparisons using RNA derived from sex determination hierarchy mutants were conducted with four replicates, with a dye-swap design; i.e. cDNA from each genotype contained incorporated Cy3-labeled dUTP in two experiments and contained incorporated Cy5-labeled dUTP in the other two experiments.

Temperature sensitive KOG1 mutant was conducted by mutagenic PCR.

Each experiment with wild-type BAP1 or F170I mutant was conducted twice.