A novel STM approach was designed to allow for a higher throughput assay of mutants using the Luminex xMAP® system.
The resulting binary and empty pCAMBIA2300 vectors were introduced into the ygl138-mediated transformation method (Liu et al. mutants using the Agrobacterium tumefaciens2007).
A new system was developed for producing conditional-lethal vaccinia virus mutants using the tetracycline controlled gene expression system from bacteria.
Saccharification performances of the tested enzymatic extracts were evaluated replacing Accelerase 1500 by each of the tested extracts (wild-type CelStrep or epCelStrep mutants) using the same amounts of units per gram, namely 5.4 units/g of pretreated biomass.
Chromosomal DNA was obtained from the selected mutants using the GENOME DNA KIT (MP Biomedicals).
Genomic DNA was isolated from overnight cultures of the mutants using the DNeasy Tissue Kit (Qiagen Inc., Valencia, CA).
This is a conclusion already tentatively suggested by ELISA on these mutants, using the MAb 105A [23].
We also analyzed the palmitoylation status of Tlg1 in sw1fΔ strain transformed with these SDP mutants, using the biotinylation assay.
We set out to characterize genomic alterations in HI mutants using the mediator-based re-sequencing approach.
We generated hos1/hos1, hos2/hos2, and rpd31/rpd31 mutants using the Tn7::UAU1 insertional mutagenesis system [24].
The slow growth phenotype and profound high-temperature sporulation defect in the ΔssrA strain explain why we were unable to recover mutants using the temperature sensitive plasmid.
