Sentence examples for clones using the from inspiring English sources

Plasmids were isolated from individual yeast clones using the Zymo Yeast Plasmid Miniprep kit (Zymo Research) and transformed into Escherichia coli for plasmid purification and sequence validation.

The data were obtained via TCRB sequencing, and are presented as the number of clones using the denoted TCRBV gene adjusted for the number of individuals (RA=65, HC=20).

The two clones derived from ATL1 turned out to be sister clones, using the TCR Vβ2.1-Jβ2 and Vα10.1-Jα15 chains.

Plasmid DNA was extracted from the clones using the Axygen Plasmid Miniprep Kit (Axygen, Hangzhou, China).

RNA was isolated from selected clones using the RNeasy kit (QIAGEN).

Plasmid DNA was isolated from individual clones using the QIAprep miniprep kit (Qiagen).

The assays were calibrated with isoform-specific RT-PCR clones using the standard curve method [36].

DNA was extracted from cell clones using the Wizard SV Genomic DNA Purification System (Promega).

To test whether this effect is cell-autonomous, we induced delg homozygous mutant clones using the Flp/FRT system.

The aligned sequences were then checked for chimeric clones using the Bellerophon 3 server at greengenes [32] and likely chimeras were removed from further analysis.

Each peak represents a group of T cell clones using the same Vß gene segment and sharing the same CDR3 length.