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A subsequent screening for structural variants using the Integrative Genomics Viewer (IGV) [ 16] enabled us to identify a 4.8-kb deletion on bovine chromosome 19 or BTA19 (g.56488278_56493087del on the UMD3.1 assembly).
These results prompted us to produce five single His-to-Ala (HA) variants using the bacterial expression system (Fig. 1).
The ability of the Drosophila transgenes to successfully rescue vps35-mutant viability allowed us to further test the relative functionality of the mutant variants using the climbing assay.
Here, we aimed to identify novel associations between IBD and functional genetic variants using the Illumina ExomeChip (San Diego, CA).
Furthermore, we analysed the variants using the same models as in the original work by Gaulton et al. [2].
Secondly, we conducted a second round of sequencing of the high-quality unique variants using the Sanger method to empirically test this prediction.
For the genes where all tested individuals of a population demonstrated AEI in the same direction (see Figure 1), it is difficult to detect any regulatory variants using the LR model.
Notably, a single group of the HIV-1 variants using the CCR5 (R5 virus [6]) predominates during the first several to 10 years or more of persistent infection in vivo [7], [8].
We annotated DNA variants using the gene models in Flybase release 5.49 [ 57].
VarScan 1.2 software [ 58] was used to identify variants using the BLAT alignments as input.
Attempts were made to rescue these 32 variants using the DENV-2 FLIC.
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