Sentence examples for mutants using an from inspiring English sources

The isolate SAKA10119 showed the best ability to adhere to HCT-8 cells, and it was therefore selected to generate transposon mutants using an EZ-Tn5™ Transposome Kit (Epicenter, Madison, USA).

We further examined the proapoptotic activities of WT and each of various Bax mutants using an ApoAlert Annexin-V kit as described [36] [37].

To determine the region of BRCA1 that confers the degradative response to γ irradiation, we generated a series of BRCA1 deletion mutants and examined the stability of these BRCA1 mutants using an in vitro protein degradation assay (Figure 4A and B) [34].

We sought to test this hypothesis by testing pre-let-7a-1 and its CTL mutants using an in vitro uridylation assay.

Subsequently, the roles played by the five transporter-encoding genes in carbon source uptake were elucidated by generating A. nidulans xtrA-E-null mutants using an in vivo S. cerevisiae fusion-based approach (see Methods).

To test for impaired function in muscle and/or neural tissue, we measured the flight performance of young ND2 mutants using an established flight assay (Benzer, 1973; Greene et al., 2003).

To confirm this we designed inhibitor-resistant (ir -Cdk2 mutants usir -Cdk2vel bioinformutantsapproach.

We produced these chimeric mutants using a similar strategy as that used for producing hTIM-1 and verified them as functional units by thermal stability assays (Fig. S4).

hip3-1 mutant was isolated by screening for suppressor mutants using a reporter plasmid.

Complementation of the mutants using a high-copy overexpression vector failed, while utilization of a low-copy inducible vector successfully restored L-form formation.

Because seam cells are responsible for producing alae [15], we examined the cell fates of hypodermal cells in acs-20 mutants using a seam cell marker (scm::gfp), which localized in the nuclei of seam cells.