Sentence examples for mutations using an from inspiring English sources

We engineered an efficient system to make Francisella tularensis deletion mutations using an unstable, poorly maintained plasmid to enhance the likelihood of homologous recombination.

Samples were sequenced for KRAS exon 2 mutations using an 189 bp amplicon.

We first assessed the kinase activities of oncogenic FGFR4K variants harboring the V550E, V550L, N535D, or N535K rhabomyosarcoma mutations using an in vitro peptide phosphorylation assay.

Similarly, targeting genes with somatic mutations using an investigational drug, requires access to a clinical trial or reimbursement for off-label use of targeted drugs with clinical outcome captured in a clinical registry study.

As described above, the E710A and Y766A mutators are impaired in the processing of at least a subset of correct base pairs, making the early checkpoints less effective in screening out mispairs so that a higher frequency of these errors can be recovered as mutations using an appropriate indicator gene (Table 6).

Furthermore, we analysed KRAS gene mutations using an ME-PCR technology whose sensitivity is 0.1% (Molinari et al, 2011), and so it is possible that the percentage of KRAS gene-mutated cells is very low in MPM and that more widely used sequencing methods are unable to detect small clones.

Notably, the SmartAmp2 method enables us to analyze mutations using a drop of whole blood and the dried blood spot.

We identified mutations using a pipeline specifically designed to accurately detect variants at very low fractions.

We screened 192 formalin-fixed paraffin-embedded breast lesions from 75 patients for point mutations using a multiplexed panel encompassing 643 point mutations across 53 genes, including 58 PIK3CA substitutions.

Patients' genotype was analyzed considering the most prevalent PM associated CYP2D6 mutations using a real-time PCR and hybridization based genotyping method.

To gain insight into the structure-function principles that govern gating and drug block, we analyzed the effect of gain-of-function mutations using a combination of two-electrode voltage clamp, single-channel recording, and outside-out macropatches.