Sentence examples for clones using an from inspiring English sources

Templates were then prepared from the shotgun clones using an automated production pipeline.

Expression of Bcl-2 was assessed by Western blotting in selected clones using an antibody supplied by Upstate Biotechnology (Lake Placid, NY, USA).

To fix the loss/replacement rate, we compared the predictions of the model with measurements of the average fraction of labeled CySCs in persisting clones using an induction frequency of 10%.

Expression of bcl-2 was assessed by Western blotting on whole cell lysates of selected clones using an antibody recognising murine bcl-2 (DAKO, High Wycombe, UK) All experiments were performed at least twice and results shown are mean of replicate samples (n=4) from a single experiment.

Q-PCR was performed on the synthesized cDNA of STC-1 and five isolated clones using an EvaGreen PCR Kit (Bio-Rad, CA) and a thermal cycler from Bio-Rad, CA. The data were analysed by CFX Manager Software (version 1.0.1035.131, Bio-Rad, CA).

Addition of a third cistron encoding YFP was shown to facilitate screening and isolation of clones using a FACS sorter.

Furthermore, we demonstrate that this 2G12 (Fab)2 display system is amenable to selection of functional clones using a mock selection.

Plasmids were purified from positive clones using a QIAprep spin miniprep kit (Qiagen, CA), and cloned in pTZ57RT cloning vector using InsTAclone PCR cloning kit (Fermentas, UK).

For broader assay applications, all 12 Env clones were converted to infectious molecular clones using a proviral backbone carrying a Renilla luciferase reporter gene (Env.IMC.LucR viruses).

Means, standard deviations, and skewness and kurtosis parameters were calculated for each tree, and compared between clones using a one-way analysis of variance (ANOVA).

DNA samples above were used to determine the copy number of the pcmdr1 (PCAS_123820) in ATN + MF-resistant clones, using a TaqMan™ probe assay.