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Here, we show that integration by lentiviral vectors can be targeted away from genes using an artificial tethering factor.
Last month, Oxford Nanopore Technologies created an industry sensation when it introduced a machine that sequenced genes using an alternative approach called nanopore sequencing, in which a strand of DNA is read as it is pulled through a microscopic hole.
We have developed an Oligonucleotide ligation assay (OLA) that enables us to screen for high-risk individuals by testing for 19 common mutations in the LDL receptor and the apolipoprotein B genes using an automated genotyping-based two-step protocol.
A pilot study of 53 patients with a panel of sequenced 35 rhabdomyolysis genes using an amplicon based sequencing panel on an Illumina MiSeq was performed.
We characterized the functional impact of these genes using an unbiased CRISPR screen of DLBCL cell lines to define oncogenes that promote cell growth.
To identify vulnerabilities relevant to specific cancer subtypes, we conducted a large-scale RNAi screen in which viability effects of mRNA knockdown were assessed for 7,837 genes using an average of 20 shRNAs per gene in 398 cancer cell lines.
Woo voluntarily retracted four papers, including two that described a method for delivering therapeutic genes using an enzyme from a bacteriophage, a virus that infects bacteria.
We further examined the predictive power of CSR genes using an alternative weighted scaling method.
We then visually-inspect these target genes using an interactive genome display tool and determine if independent evidence for alternative splicing or polyadenylation is available.
To characterize the mechanisms of IL-2-induced T cell proliferation we sought to identify new IL-2 target genes using an Affymetrix array chip (Mu19K).
We generated individual gene trees for each of the 69 virulence genes using PhyML [38] and looked for the rejection of a set of these topologies by the 69 genes using an Approximate Unbiased (AU) test [39].
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