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Model plausibility was evaluated using a kinetic simulator [7], [10] that allowed us to define the transcription process in terms of elementary steps that were relevant to the mutants under study.
The quorum sensing mutants under study exhibited expected bioluminescence phenotypes, in keeping with previous findings e.g. the HAI-1-mutant produces 0.3%, the AI-2-mutant produces 3% and the CAI-1-mutant produces 33% of the light produced by the wild type, respectively (see Figure 4).
Crystallization of mutants under study was carried out as described previously.
This discovery highlights the existence of distinct mutations unique to the muscat-like aromatic mutants under study, as opposed to the SNP found in Muscats.
Nevertheless, the lifespan of Δ 3'pex5 cells is still clearly reduced relative to WT cells and the shortest among all pex mutants under study, indicating that the defect in PTS1 protein import has a strong negative effect on chronological aging.
The putative causal SNP identified in Muscat varieties as well as the unique mutations identifying the muscat-like aromatic mutants under study may be immediately applied in marker-assisted breeding programs aimed at enhancing fragrance and aroma complexity respectively in table grape and wine cultivars.
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Additionally the authors should state online as part of the results given what threshold values are used to conclude based on the dynamics that any of these proposed mechanisms are not favoured in the mutant under study.
In addition to using a different cell type, these experiments differ from those carried out with S2 cells by Izzo et al. [ 38], in that endogenous wild-type MLE was absent in the transgenic flies examined, and thus did not compete for inclusion in the MSL complex with the mutant MLE protein under study.
Moreover, we can compare the wildtype protein's unfolding pathways with mutants of the protein under study, or we can study the effect of a ligand.
Engineering fluorescently labeled genes of interest in a fosmid context also potentially allows for evaluation of the functionality of the tagged gene as it should rescue the mutant phenotype of the gene under study.
The aisles had once swarmed with people, mutant under-dwellers who emerged from the depths of Penn Station.
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