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The counterpart tags are attached to the genes under study.
The M.H.C. genes under study varied at seven sites, so a range of matches was possible.
Forward and reverse primers of the genes under study.
Apart from some expectations, all the genes under study exhibited also different transcription ratios under deficit stress.
It is commonly used in reporter gene assays, where the promoters of genes under study have been linked to the luciferase gene, which in the presence of its substrate (luciferin) generates light.
Primers for the genes under study were designed based on cervid sequences or on conserved regions of Bos taurus sequences when cervid sequences were not available.
Hence, we hypothesized that DTT could accelerate Fe3+ reduction, thereby modulating cellular iron traffic and, consequently, regulating the expression of the genes under study [reviewed in 20].
The probability of success in a single Poisson trial can be calculated by multiplying the list-specific occurrence probabilities for the combination of genes under study.
The 13 species of Drosophila exhibit at least 419 computationally identifiable HSEs in the 8 heat-shock genes under study (Table S1, Table S2, Figures 2 9).
Serial dilutions of all cDNAs were previously amplified to establish the appropriate dilution to attain a detection range between 10 and 35 cycles for all endogenous and genes under study (1/50 dilution).
The finding that functional meiosis-related gene duplications go to fixation at the same rate as the average for all genes is surprising, especially in the light of the complex roles that the genes under study perform (see Table S1).
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