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The growth rate of populations derived from different mutants that arose among the bacteria cultured with rif was significantly different, according to a one-way analysis of variance (not shown here).
In addition to the isolation of several zebrafish chromatophore mutants that arose spontaneously [12], [13], numerous mutagenesis screens have yielded over 100 mutations affecting various processes in the development of different combinations of the chromatophore types [6], [14] [17].
We then relate the net growth of mutant cells to the net growth of sensitive cells via the relative fitness (see Supplement S1); the mean mutant population at the time of detection is the sum of the descendants of all of the mutants that arose during the growth of the sensitive population.
The fraction of induced mutants that arose by this mechanism depended upon the inducing agent.
Thus revertant colonies were initiated by mutants that arose under nonselective conditions, either before plating or during nonselective growth on day 1. (We will provide evidence below that roughly half of the day-6 colonies are initiated before plating and half early during day 1).
Similar(55)
The model will utilize experimental data on the types of mutants that arise in the context of different treatments.
These results (seen in Figure 9) support the idea that revertant colonies are initiated by mutants that arise during nonselective pregrowth in liquid medium.
Defective interfering (DI) viruses are natural mutants that arise spontaneously, occur widely, and have a genome that has undergone at least one major deletion.
The exceptional selection systems that originally suggested stress-induced mutagenesis may also be explained entirely by natural selection, acting on mutants that arise at standard mutation rates.
KNOWING the source of mutants that arise under selective conditions is critical to understanding evolution and therefore the origins of cancer and the progress of infectious disease.
Evidence is presented that the accumulated Lac+ revertant colonies are initiated by small-effect (weakly Lac+) mutants that arise prior to selection and improve by acquiring a second mutation during growth under selection.
Related(20)
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