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The new data are consistent with the original model and provide new mutants that allow one to ascribe certain features to the linker, and not decoupling.
This conservation of regulatory mechanisms when human HSF1 is expressed in yeast allowed for the development of a facile assay system to identify human HSF1 mutants that allow human HSF1 complementation in yeast.
The fruit fly D. melanogaster and the roundworm C. elegans have been essential model organisms for studying development and disease, in part because it is relatively easy to create mutants that allow the functions of individual genes to be investigated.
To provide support for these linkages on LRRK2, we used an expression system with ubiquitin mutants that allow conjugation through only one lysine residue, Lys or Lys, whereas all other lysines are mutated to arginine.
Despite the great advantages of the insertion mutants that allow the recovery of the genomic sequences adjacent to the integration sites of the transposons [ 44], the genomic resources of M. truncatula also renders the rapid identification of deleted genes by either map-based cloning or transcript-based cloning, as successfully demonstrated in several recent papers [ 30, 38].
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Here, we describe quantitative DNA tags (qTags) and their use to simultaneously analyze conditional Mtb knockdown mutants that allowed silencing the glyoxylate and methylcitrate cycles (via depletion of isocitrate lyase, ICL), the serine protease Rv3671c, and the core subunits of the mycobacterial proteasome, PrcB and PrcA.
Two of the other four TFs that were found to be down-regulated under ER stress (NCU09549, NCU04848, NCU08923, and NCU07430 had available mutants that allowed us to test their enzyme production.
To construct a comC mutant that allowed us to assay CSP-dependent bacteoricin production, we transformed the previously generated comC deletion construct [ 20] into S. mutans GS5, a wild-type strain that was demonstrated to produce all known QS-controlled bacteriocins [ 6, 7, 22].
The quasispecies is a population structure typically formed at high mutation rates that allow the mutants to stay closely connected and thus be easily regenerated from one another even if lost from the population through random genetic drift.
In order to define the Ub chain linkage of Beclin 1, we expressed HA-tagged versions of single lysine mutants of Ub that allow the formation of Ub chains via one lysine residue only.
Advances in mutant RNAP simulation technology that allow analysis of many more mutant proteins in different contexts would be very useful for future studies.
Related(20)
deployments that allow
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shifts that allow
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com