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Exact(5)
Ectopic bristles are also observed when hyperactive Mad mutants are overexpressed in the fly wing [6].
This is an interesting question and points to the limits in interpreting experiments in which the kinase inactive mutants are overexpressed.
First the evidence shown in Figure 4D is not sufficient to say that "telomeres shrink" when the TEN domain mutants are overexpressed.
In addition, a small proportion of primary neuronal cells shows cytoplasmic localization, which is enhanced when TDP-43 mutants are overexpressed [ 13, 54, 73, 99].
Success with genetic screens to identify suppressors and enhancers of disease when mutants are overexpressed in a developing tissue, such as the eye-antennal imaginal disc, suggested to us that it might be possible to generate a fly model of misfolded insulin-associated diabetes.
Similar(55)
HbpR and HbpR mutants were overexpressed and purified from E. coli.
These RelB deletion mutants were overexpressed in HEK 293 cells together with HA-tagged RelA.
WT and each of these Bax mutants were overexpressed in Bax−/− MEF or lung cancer H157 cells and the proapoptotic activities were compared.
To directly verify that a phosphorylation of NPM/B23 on S4 promotes centriole duplication, NPM/B23 mutants were overexpressed in unperturbed U2OS GFP-Centrin1 cells.
GST-YY1, full length or various deletion mutants, was overexpressed in bacterial cells, and then bacterial lysates were used in a radioactive in vitro kinase assay with purified Plk1.
Human wild-type α-Syn and the A30P, E46K and A53T mutants were overexpressed in E. coli BL21 DE3) cells using plasmid pT7-7 and purified as described previously [81] with minor modifications, as follows.
Related(18)
variants are overexpressed
genes are overexpressed
mutants are used
mutants are summarized
mutants are derived
mutants are described
mutants are affected
mutants are generated
mutants are shown
mutants are listed
mutants are obtained
mutants are found
mutants are born
mutants are reported
mutants are seeded
mutants are threatened
mutants are rounded
mutants are called
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