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In all cases, solutions that kill all the non-equivalent mutants are obtained.

Less than 10% of homozygous N-myc mutants are obtained from heterozygous crosses as previously discussed in (Dominguez-Frutos et al., 2011).

In cases where monoallelic mutants are obtained, it is possible that the mutant line could be maintained and embryos reinjected with TALEN mRNAs to induce biallelic mutations.

The osphyA osphyB double mutants were obtained by crossing osphyA-2 and osphyB-2 single mutants.

These expected results were observed (Fig. 2a), indicating that Δtri3 mutants were obtained successfully.

Finally, nine suppressor mutants were obtained (Sup13, Sup14, Sup15, Sup16, Sup17, Sup18, Sup19, Sup23, and Sup24).

More than 100 mutants were obtained from Agrobacterium sp. ATCC 31750 strain by chemical mutagenesis.

Through site-specific mutagenesis, several improved mutants were obtained, with GadB1E312S being the best one.

Via this method, four new fluorescent protein mutants were obtained based on monomeric cherry fluorescent protein.

By screening 400 clones from random mutant libraries for enhanced fibrinolytic activity, two mutants were obtained: N111R, R230G.

The following mutants were obtained: Asp170Glu, Gly178Ser, Leu218Ser or Thr, Arg238Val, Thr245Ala or Ser and Ser264Lys or Ile.