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However, Ub K-to-R variants are overexpressed in living cells, and the presence of wild-type Ub often complicates analysis.
In humans, RAGE and RAGE splice variants are overexpressed in adenocarcinomas of the pancreas and correlate with proliferation and invasiveness (15).
Importantly, all hTERT variants are overexpressed relative to endogenous telomerase (see western, Figure 4A).
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Five mouse Optn variants were overexpressed under the CMV early enhancer/chicken beta-actin (CAG) promoter in transgenic mice.
Wild-type and mutant Mt-PolDom variants were overexpressed and purified as described (Pitcher et al., 2005).
WT-FIH and all variants were overexpressed in Escherichia coli with an N-terminal His6 tag and purified as previously described.
The dSfmbt-4MBT protein and all variants were overexpressed in Escherichia coli strain BL21 DE3) as TEV-protease-cleavable N-terminal His6-fusion proteins at 18°C for 15 h.
18 20 In this study, we revealed that both of the ADAR1 p110 and p150 variants were overexpressed in approximately 70% of the primary HCC samples whereas ADAR2 was downregulated in approximately 50% of HCC cases.
This gave the expected results and furthermore showed that the reconstituted TrxR1 variants were overexpressed with regard to the endogenous TrxR1 level in the parental Txnrd1 fl/fl cells.
These effects are more significant when the Ser variant is overexpressed: the pAKT increase after overexpression of the Cys variant is nonsignificant.
Strikingly, in comparison to overexpressed wild-type TPI, a strong reduction in the endogenous TPI level was detected in case the Glu104Asp variant was overexpressed (upper panel).
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