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Exact(5)
As was predicted by flow cytometry, chains of the dltA mutant showed very similar levels of C3 signal within chains and from chain to chain (Fig. 5 B).
Our functional studies demonstrated that CupB4, like the CupB2(R36A) mutant, showed very little binding towards the C-terminal carboxylate of CupB6.
The K175L mutant showed very similar thermostability to wild-type αB-crystallin with precipitation occurring in two phases at 68°C and 78°C.
Interestingly, the R140K mutant bound the leading substrate, DCoA reasonably well with ΔH and Ka values similar to those of the WT (Table 2), but this mutant showed very poor binding to ATP, thereby explaining the loss of activity for this mutant.
A deletion mutant for the histidine kinase encoding gene vicK which responds instantaneously to carolacton treatment together with all the vicR-coexpressed genes, was similarly tested for carolacton-sensitivity, but since the vicK mutant showed very poor biofilm growth, further inferences could not be made.
Similar(55)
Given that the Y766F mutant shows very little difference from WT in terms of reaction kinetics, fidelity, and other properties that have been examined in this and previous studies, it is perhaps surprising that tyrosine is invariant at this position in A-family DNA polymerase sequences.
In fact, the latter seven mutants showed very marked attenuation with an LD50 of ≥107 CFU.
However, Ndc80Δ490-510 and Ndc80-7A mutants showed very little interaction with Dam1.
Fig. 6B and Supplemental Fig. 5 demonstrate that the single, double and triple mutants showed very similar and wild-type-like far-UV ECD spectra with two prominent minima at 218 nm and 229 nm [6].
More importantly, upon H2O2 treatment CHIP WT-expressing cells showed weak or no TUNEL staining, whereas cells expressing the deletion mutants showed very bright TUNEL staining equivalent to that of vector-transfected cells, indicating that EndoG levels correlated with the incidence of oxidative stress-induced cell death.
Delg mutants show very similar phenotypes compared to normal fed controls, and do not show additive phenotypes in respect to mitochondrial abundance and amino acid metabolism upon low-yeast food.
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