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The double mutant produced very few fruiting-bodies after 21 days of growth, and they accumulated only on a few distinct positions of the plate (Figure 7C).
As depicted in Fig. 1D, the JSK3 suppressor mutant produced very small plaques compared to ampAOE.
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Meanwhile, Y15A-V5, K186Q-V5, or LL241 242AA IN mutants produced very low levels of the dimer or multimer forms under the same experimental conditions.
In fact, the group inoculated with the mutant strain produced very low quantities of IFN γ after PPDB stimulation throughout this study).
The adhE1 mutant in particular produced very little solvents, demonstrating that this gene was indeed the main contributor to ethanol and butanol formation under the standard batch culture conditions employed in this study.
Figure 6A shows three independent tests performed with the zmpA mutant that all produced very similar mean indices, confirming the reproducibility of the approach.
The mutant band 3 produced very little chloride influx when expressed in Xenopus oocytes.
We therefore proceeded to express the respective SPA coding sequences under the control of the endogenous Arabidopsis AtSPA1 and AtSPA4 5´ and 3´ regulatory sequences which previously produced very high complementation rates among transgenic spa mutant plants (>90%) [ 27, 52].
The prsD (A898) mutant lacked extracellular glycanase activity, failed to form biofilm rings in shaking culture, and produced very mucoid colonies and viscous culture supernatants (not shown).
However, the Arabidopsis 35S::andPA1 and 35S::AtSPA4 constructs produced very low complementation rates (<10% of transgenic plants) in the spa triple mutant, an observation we had made before [ 52].
In Scrambled mutants displaying abnormally small actin caps but normal prophase spindle length in late prophase, myosin II inhibition produced very short spindles.
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