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Since in both the Sytox Green® and luciferase assays PA14 and the ΔgacA mutant behaved very similarly, we checked gene expression patterns in the host.
In addition, the W38C mutant behaved very much like the wild type in terms of the dimer state, suggesting that RsTSPO naturally forms a dimer by strong associations other than disulfide bonding.
Inactivation of F3 by a C298A mutation yielded soluble protein, but to our surprise the mutant behaved very similar to wild-type PARP-1 with respect to the length dependence of stimulation and inhibition of the sumoylation reaction (Fig. S8).
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Neither the nrdEF (PI = 1.204), nor the nrdDG mutant (PI = 1.585) had significant differences when compared to the wild-type strain (PI = 1.239), and the nrdAB mutant (PI = 0.143) behaved very much alike in both cell lines (Figure 7A).
Therefore, even the occasional lamellipodia that appear morphologically normal in SCAR Δ37 mutant hemocytes behave very differently to wild-type lamellipodia and this altered behaviour can be dramatically rescued simply by removing apoptosis, further highlighting the delicate balance between the machineries controlling phagosome processing and migration.
The Δflv4 strain behaved very similar to the Δflv2 mutant (data not shown).
"I behaved very badly.
"They behaved very badly.
"Dominic always behaved very well.
"I think Megyn behaved very nasty to me," he said.
(Afterward, Trump complained that Kelly "behaved very badly").
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com