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In our study we used the strain CC503, a nit1 nit2 mutant lacking an active nitrate signaling pathway (nit2).
For controls, culture supernatant from the wild-type strain and an inactive T6SS mutant lacking an essential component, Δ tssE, was also probed.
Moreover, the ycf1Δ mutant lacking an ABC-type transporter responsible for sequestration of metal-glutathione conjugates into vacuoles [ 27- 29] was also As and Cd sensitive.
In this regard, our data suggest that in mammals KPNB1 mediates nuclear localization of the PER/CRY complex independently of importin α, as overexpression of a KPNB1 mutant lacking an importin α interaction domain had a similar phenotype.
Similarly, the data of Ding et al. [ 100] showing increased dimer level in the S505N mpl mutant lacking an ECD would be in this case explained by the short DSG linker (7.7 Å) crosslinking the amino-terminal residues more effectively if the mutation had altered helix packing to the State 2 orientation.
Similar(55)
Conversely, the truncation mutant lacking a portion of the fusion domain was unable to promote these steps.
Typhimurium A9 (mutant lacking a 15kB fragment spanning recA-hydN genes) formed more abundant biofilms, which were not responsive to treatment with nitric oxide donor molsidomine.
Kane, WH, Devore-Carter, D, and Ortel, TL. "Expression and characterization of recombinant human factor V and a mutant lacking a major portion of the connecting region". Biochemistry 29, no.
Interestingly, these mAbs changed their binding also to other mutant, lacking a transmembrane anchor-Gln1230Stop.
Similar experiments could be done for transverse vs. axial motion comparing wild type to model organisms such as the myosin-XVa mutant lacking a staircase architecture.
The Arg175Gln mutant (lacking a positive charge) activity was also linear with bicarbonate concentration (Fig. 4).
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