Sentence examples for mutant containing an from inspiring English sources

The construct pET28a MsrB1-Cys, described previously [22] was used for expression of MsrB1-Cys mutant containing an N-terminal His-tag in E. coli BL21 (DE3).

These peptides have properties similar to apoA-I Milano [17], [43], a naturally occurring apoA-I mutant containing an extra cysteine disulfide bridge.

The main chlamydomonas strains exploited were IL, a mutant containing an intronless psbA gene [46] that represented the control reference strain for characterization of the D1 mutants, and Del1, a mutant used as a recipient strain for D1 mutants generation.

To determine whether the presence of cysteine residues is sufficient to allow for functional activation in the absence of tyrosine phosphorylation, we used a STAT1-CC mutant containing an Y701F substitution.

This strain was tested in a competitive index experiment against a derivative of the ΔSPI-19 mutant containing an empty copy of the vector, as well as the wild-type parental isolate bearing the empty vector (strains ΔSPI-19/R995 and WT/R995, respectively).

Nevertheless, when the RAW264.7 cell line was treated with 65 µM of the iron chelator 2,2'-dipyridyl (DIP), the nrdAB mutant containing an additional nrdHIEF copy showed a significantly higher PI than that of the untreated assay or the RAW264.7 Nramp1+/+ cell line (Figure 7B).

An hCaM mutant containing a unique cysteine residue at position 124 on the protein was expressed, purified, and chemically modified with the fluorophore monobromobimane (mBBr).

Mutations at three positions were introduced to the XYNII mutant containing a disulfide bridge (S110C N154C) in the α-helix.

Here, we present a lipase mutant containing a biochemical switch allowing a controlled opening and closing of the lid independent of the environment.

Surprisingly, the GAM-proghrelin mutant containing a Ser6 to Ala6 mutation was octanoylated, while the Ser5 to Ala5 mutant was not (Figure 7C, lanes 2 and 3).

This pattern was previously observed in cells expressing the inactive Gap1-92 mutant containing a single glutamate-to-lysine substitution at position 300 and shown by membrane fractionation to be stacked in the ER [4].