Sentence examples for mutant using an from inspiring English sources

pestis or an isogenic irp2 mutant, using an artificial feeding device, and maintained as previously described [7].

A Southern blot was performed on genomic DNA from the Ccel_1894 mutant using an intron-specific probe.

For synaptic modeling, we aligned 10 consecutive sections of an acetylcholine neuron from apm-2 apa-2 double mutant using an imageJ plugin called TrakEM2 (Cardona et al., 2012, http://www.ncbi.nlm.nih.gov/pubmed/22723842).nih.gov/pubmed/22723842

We previously constructed an HcDPPIVA-deletion mutant using a Ku-deficient strain of H. capsulatum.

We mapped the 88.21 mutant using a CA panel and identified a mutation in the catalytic domain of the aldh1a2 gene; therefore we named our mutant aldh1a2um22.

Although we created this mutant using a clathrin cryo-electron microscopy model and a yeast temperature-sensitive mutant as a guide, the resulting temperature-sensitive clathrin showed an altered phenotype compared to the corresponding yeast temperature-sensitive clathrin.

We further analyzed the function of the D-CblL-mPR mutant using a constitutive expression promoter, HS83 [40], which was also used in rescue assays by D-CblL and D-CblS.

Next, we sought to selectively elevate myosin activity in a fra mutant using a constitutively active Myosin Light Chain Kinase (U-ctMLCK) that increases myosin activity by phosphorylating the Regulatory Light Chain of myosin II [56].

We also performed transgenic rescue of the bub-1 mutant using a PCR product which encompasses the region from 1.40-kb upstream to 0.82-kb downstream of the bub-1 locus.

To pinpoint the defect in daughter abscission at the molecular level we evaluated the development of the cytoskeleton in the MORN1 mutant using a battery of markers to highlight different aspects of the budding, maturing and mature cytoskeleton (Fig. 5).

As for peg2 no additional alleles were available, we complemented the peg2 mutant using a genomic construct.