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In contrast, the CLC mutant generated in this study was resistant to the bactericidal action of guinea pig or human serum.
SdcBG02774 is a mutant generated in the w1118 Canton S (B) [CS (B)] strain by the insertion of a p[GT1]-element in the second intron of dSdc (Fig. 2A) [2A].
At the same time, our previous results focusing on the impact of nuclease production on biofilm formation in UAMS-1 were done using a mutant generated in the nuc gene designated as open-reading frame SA0746 in the N315 genome, mutation of which was shown to abolish nuclease production even in a UAMS-1 sarA as assessed using DNase agar [23].
This is in contrast to the EP/GPEET null mutant generated in bloodstream forms, which makes the transition from a full VSG coat to no glycoprotein coat when the parasite differentiates to the procyclic form [20] or to the Nour 6C mutant [21], which first becomes negative for procyclins when GPEET is repressed a few days post infection in the fly midgut [16].
The corresponding pea sequence was obtained from JI 2822 (Fig. S4) and primers designed to the 3' end of the gene failed to generate an amplicon from FN 3690, a white flowered fast neutron mutant generated in the line JI 2822 [24]; this mutation is allelic to a2.
Another Vps1 mutant generated in the study was vps1 E461K.
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All the single, double and triple mutants generated in this study were verified by PCR (Additional file 1: Tables S1, S2).
Unlike E. coli mutant, the four R. eutropha mutants generated in this study (1F2, 6C1, 12D1 and 13H3) did not secrete leucine to the culture medium, as revealed by HPLC analysis (data not shown).
Additional mutants generated in this study were the N-flagged PLN with R13E mutation, R13A, R13E/R14E double mutant.
Thus the majority of our mutants generated in crosses 1 and 2 (see Table S1) were dpolv, dpol, and dplv in decreasing order.
In general, we obtain greater yields of macroconidia when using this approach instead of the standard method of growth in liquid CMC media (also, some of the mutants generated in our laboratory do not sporulate well in CMC).
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