c) We identified variants generated in b) in which maximised the increase in identifiers with cardinality = 1.
On this basis, any difference in sequence variants between the cloned and genomic DNA templates from the same individual is highly likely to reflect 'biological' sequence variants generated in vivo and not technical artifact arising from the sequencing process.
Opposite G and four DNA lesions, the R96G variant generated extension products considerably lower in extent than the wild-type in the presence of Mn2+, as similarly observed with Mg2+, although the decrease of R96G bypass extent was less opposite template N-EtG than the other templates.
Human variant databases support the aggregation, curation, and sharing of data on disease-associated variants.1,2,3,4 Variant databases not only curate the literature but also facilitate access to unpublished variant classifications generated in diagnostic laboratories.
The mouse-adapted variant was generated in the serial passage experiments described Bell et al [21].
An oestrogen regulated protein in osteoblasts, KLF10 is another member of the Sp/Krüppel-like factor family that has two amino-terminal variant isoforms generated in a similar way to those of Sp8.
The weak [PSI+] phenotypes of some strains (e.g. A4) could be caused by weakened propagation of the same prion variant or by the formation of a different prion variant To test the possibility that different, structurally distinct, prion variants are generated in the presence of different Hsp70 orthologs, we focused on A1 and A4 that have normal and weak prion phenotypes, respectively (Figure 5A).
Because of this adaptive process, epitopes presented by common HLA variants are generated in low amounts, whereas epitopes presented by rare HLA variants are made in abundance.
All variants were generated in MGBR2_ex19 27 and assayed in HeLa cells.
Variants were generated in which residues in these clusters were replaced with either Tyr or Ala.
