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Thus the phenotypes of IFT null mutant embryos do not reveal any elevation of canonical Wnt signaling in the absence of cilia or when cilia structure is disrupted.

IFT mutant embryos do not show the craniorachischisis (open neural tube caudal to the forebrain) characteristic of mouse mutants that lack components of the non-canonical Wnt pathway [32], [33], suggesting there is not a strong disruption of non-canonical Wnt signaling in IFT mutants.

(D ) Kif5aa *162 mutant embryos do not show an optokinetic response.

Lefty1 RNA-injected M ybx1 sa42 mutant embryos do not manifest cytokinesis failure or YSL defects even at the restrictive temperature.

neurog3 homozygous mutant embryos do not display any modifications in the number of pancreatic endocrine α-, β-, δ- and ϵ-cells compared to wild type embryos.

DOI: http://dx.doi.org/10.7554/eLife.02443.011 10.7554/eLiFigure4 figureigure 4—figure supplement 2. His C mutant embryos do not show accumulation of DNA damage during early embryogenesis.

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Analysis of HS treated embryos revealed that transgenic overexpression of Tg(hsp70l:unplugged SV1-myc) in wildtype or unplugged mutant embryos did not affect the location of the AChR prepattern (Figure 5A F), or motor axons guidance (Figure 5I and J).

h1 and hIJ3 mutant embryos did not show defects in formation of the primary tracheal branches (data not shown); however, in embryos trans-heterozygous for h22 and h1J3, the DT was broken (data not shown).

However, untreated His C mutant embryos did not exhibit detectable levels of pGRP, which we confirmed by Western blot analysis.

(F and G ) His C mutant embryos did not degrade Cyclin B and failed to accumulate string mRNA.

Despite the high expression level of the DAG2 gene during embryo and seed development, microscopic analysis of dag2 mutant embryos did not reveal any noticeable phenotypical alteration.

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