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Lower bacterial loads indicate that the overall less virulent mutant bacteria do not survive as well in the blood and therefore may not even reach the brain.
Calves challenged with intimin-deficient mutant bacteria do not develop diarrhea or attaching/effacing lesions, nor are colonized to the same extent as animals infected with wild type or complemented mutant strains [ 20].
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In addition, once internalized the number of mutant bacteria did not significantly increase eight hours p.i. (see Supporting Figure S1), which indicates that either the mutant bacteria replicate more slowly than the parental strain or are killed by the host cell at a rate that is approximately equal to their rate of replication.
Supernatants of Δ scpA mutant bacteria did not convert the substrate and complementation of the mutant restored activity to wild-type levels.
Incubation of IgM with wt Cc5 bacteria determined a dramatic reduction of SNA staining and a size shift of the IgM heavy chain indicating deglycosylation while incubation with PUL5 mutant bacteria did not affect the glycosylation state of IgM showing that IgM deglycosylation by Cc5 is only PUL5 dependent.
"Bacteria don't always decide.
(Bacteria do not have nuclei).
However, bacteria do not express geminin.
Interestingly, the nrf-5 mutants fed HT115 bacteria did not have increased lifespan.
Moreover, the presence of wild-type bacteria did not enhance the growth of the signal-defective lasI mutant.
She didn't invent CRISPR; bacteria did.
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CEO of Professional Science Editing for Scientists @ prosciediting.com