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Thus, Lerp F6 mutant flies do not express detectable LERP protein.
Nevertheless, dj-1β mutant flies do not display decreased lifespan, which again is in contradiction with MFRTA.
However, no obvious morphologic defect was observed in the wing or scutellum when faf RNAi was misexpressed in the wing disc by the C96-Gal4 or dpp -Gal4, which is consistent with the observation that faf mutant flies do not display any abnormalities in the wing or the notum (Overstreet et al. 2004).
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This increased susceptibility was local and thorax specific, as the survival kinetics of abdominally injured and infected wild-type and mutant flies did not differ (Fig. 2B and Table S2).
This hypothesis is supported by the observation that, despite reduced dilp2 transcript levels, male dSdc mutant flies did not show a compensatory increase in dilp3 mRNA as a result of the reduced insulin signaling in the IPCs, an autocrine regulatory mechanism proposed by previous studies [34], [48].
Hence, the increased mitochondrial ROS seen in mutant flies did not increase ROS-generated macromolecular damage (Fig. S8).
Dgp71WD mutant flies did not display uncoordinated behaviour, and centriole number was not detectably perturbed in larval neuroblasts (supplementary material Fig. S1).
Of note, the analysis of mutant flies did not reveal a role for dPSR in the phagocytosis of apoptotic cells during development.
Mutant Eater non-primed flies were more susceptible to infection than OR non-primed flies (P<0.0001), and contrary to wild type flies, priming of mutant flies did not lead to any survivors at 30 hours.
The fact that young per mutant flies did not show impaired climbing demonstrate that the period gene does not affect fly geotaxis per se, but rather contributes to impaired climbing ability in an age-dependent fashion.
We verified that the decrease of OPMD phenotypes in bb N /Df(1 17-87 mutant flies did not result from a lower production of PABPN1-17ala in this context (Fig 6D).
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