Sentence examples for mutant and empty from inspiring English sources

Src cDNAs (pUSE Src wild type, pUSE Src kinase mutant, and empty vector) were obtained from Upstate Biotechnology, Inc. (Lake Placid, NY).

The HSC3 cells were transfected with the pEGFP-SHP2 WT or the SHP2 C/S mutant and empty vector, and harvested for use in the immunoprecipitation assay.

No difference in p38 phosphorylation has been reported between mutant and empty vector cells in response to drug (data not shown).

The HSC3 cells were transfected with the pCMV Tag 2B-SHP2 wild type (WT) or the SHP2C/S mutant and empty vector by using a lipofectamine reagent (Life Technologies), according to the manufacturer's protocol, and then subjected to invasion, metastasis assays and western blot analysis.

Equally, when we assayed the transformed yeast microsomal protein fractions in vitro for DGAT activity using unlabeled oleoyl-CoA (18 1) as an acyl donor, and 14 C- sn-1,2 diolein (18 1) as acceptor, enzyme activity was not detected in the yeast strain harboring the mutated DGAT1 cDNA from the AS11 mutant and empty control pYES2.1 vector, but was found in the positive WT control.

Immunoblots probed with antibodies that recognize all three major carboxysome shell proteins (CsoS1A, CsoS1B and CsoS1C) clearly showed that the CsoS1 proteins were present in all mutant carboxysomes and empty shells at approximately the same levels as in the wild type (Figure 7A,C).

HeLa cells were transfected with plasmid MTS- hOGG1, MTS-mutant hogg1, Nuc- hOGG1, Nuc-mutant hogg1, and empty vector (Invitrogen, Carlsbad CA) using Fugene 6 (Roche, Indianapolis IN) in the ratio of 1 6 (DNA in μg: Fugene in μl).

Western blot analysis of the cells transfected with the dominant negative p85 mutant and the empty vector confirm Δp85 expression in these macrophages (Fig. 2A).

Plasmid constructs used in transient transfection include EGFP-C1-WT α-syn, pEGFP-C1-A53T mutant α-syn and empty vector pEGFP-C1; pcDNA-SIRT1 WT and mutant SIRT1 H355A; pRC-CMV-NEDD4 WT and NEDD4 C866S; HSF1 WT; HA-Ub.

Ectopic expression of the mutant Y477F ezrin and empty pCB6 vector in AC2M2 cells is previously described (23).

H441GL cells (CL1-5F4 A549A549 cells) were seeded at 5 × 105 cells in 6-cm plates and were transduced with wildtype pcDNA3.1/MKP-1, domutant mutant pcDNA3.1/MKP-1CS (C258S mutandon) and empty pcDNA3.1 vectors using Lipofectamine 2000 (Invitrogen, Taipei, Taiwan).