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e Comparison of grain weight between gns4 mutant and complementary transgenic plants.
d Comparison of grain number per panicle between gns4 mutant and complementary transgenic plants.
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These results demonstrated that the ΔrovA mutant and the complementary strain ΔrovA-pAraRovA were successfully constructed.
To confirm the successful construction of the ΔrovA mutant and the complementary strain, the parent strain, the ΔrovA mutant and ΔrovA-pAraRovA were grown in BHI broth at 26°C to stationary phase, when maximal rovA expression has previously been shown to be achieved [21].
These two plasmids were used for transformation of B. licheniformis WX-02 for making the budC deletion mutant and its complementary strain, respectively.
c RT-PCR of REL2 in rel2 mutant and three independent complementary lines c-rel2-1, c-rel2-2 and c-rel2-3.
H. capsulatum Δags1 mutant and its isogenic complementary strain AGS1 were derived from the clinical isolate G186A (ATCC 26029) and have been described in ref 39.
Mutation of the GAS site (5'-TTCCAAGAA-3') within the distal LKB1 promoter region at -1152 was accomplished using the Site-Directed Mutagenesis kit (Stratagene) and complementary mutant oligonucleotides corresponding to the sequence 5′-CCAGCATTATCTCCAGA TTagtttAAGTTGGGGTGTGAGCCAG-3′ (the GAS site is italics; mutated base pairs in lowercase letters).
Taken together data from these multiple and complementary mutants point toward the presence of a network of positive and negative regulatory elements, with the natural missense changes mapping an ESS sequence down-regulating the adjacent cryptic 5′ss.
The wild strain WX-02, mutant strain WX-02 Δ budC, and complementary strain WX-02 Δ budC/pHY budC were cultured for 12, 24 and 36 h.
Three different types [RRE IIB RNA (complementary), RRE IIB mutant and T7 primer DNA] were evaluated by DPV measurement.
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