Sentence examples for mutagenesis efficiency of from inspiring English sources

We also tested the mutagenesis efficiency of sgRNAs with mismatches to the intended targets.

The ratio of the cleaved fragments to the uncleaved fragments reflected the mutagenesis efficiency of the CRISPR system.

The mutagenesis efficiency of ZFN-induced gene targeting in Drosophila has been estimated to be as high as 1 10% at several loci including ry, coil and pask genes [ 47, 48].

To examine the mutagenesis efficiency of Cas9 nickase, we constructed a series of sgRNA vectors to target the white (w ) gene and introduced pairs of them into P{nos-Cas9 D10A}attp2 embryos by co-injection.

However, the mutagenesis efficiency of an sgRNA pair with the Cas9 nickase is at least two-fold lower compared with either one of the sgRNA pair using the wild-type Cas9 (Table 1).

Then, T7 endonuclease I (T7E1) mutation detection assay was employed to determine the mutagenesis efficiency of the Cas9/I2-1 system on the I2-1 candidate site and its 8 putative off-target sites.

Both somatic and heritable mutagenesis efficiencies of TALENs were higher than those of the CRISPR/Cas9 system that we recently developed.

The somatic and heritable mutagenesis efficiencies of TALENs were higher than those of the CRISPR/Cas9 system, although optimal concentrations of gRNAs and Cas9 mRNAs need to be further examined.

To compare mutagenesis efficiencies of different sgRNAs, we designed a second sgRNA targeting the yellow gene (y2) and an additional two sgRNAs targeting the second (w1) or third (w2) exons of the white gene.

Importantly, we document for a strong positive correlation between mutagenesis efficiency and sgRNA GC content of the six protospacer-adjacent motif-proximal nucleotides (PAMPNs).

In the absence of further statistical data, we speculate that the mutagenesis efficiency might be correlated with the nucleotide composition or the epigenetic state of target regions.