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The mutagenesis data of these two lesions in two different mammalian cell lines indicate a high error rate not only opposite the lesion but also in the local region, which suggests involvement of an error-prone Y-family DNA polymerase in TLS.
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The homology model of the IL-3:receptor complex generated in this study provides a molecular framework to analyze a large body of mutagenesis data on the roles of specific residues in IL-3:receptor complex formation and function.
The structural features of interface II are consistent with mutagenesis data on the functional roles of interface residues (Figure 1C).
The observed binding mode is consistent with most of the mutagenesis data on this complex.
Together with our previous mutagenesis data on ICL2 and ICL3 we provide here the first systematically completed map of potential interfaces between TSHR and heterotrimeric G-protein.
The publication of the crystal structures of zebrafish P2X4 receptor in inactive and ATP-bound active forms provided structural data for the analysis of the receptor structure, the interpretation of mutagenesis data, and the depiction of ligand binding and receptor activation mechanism.
Two types of mutagenesis data indicated that the Anc1-containing branch of the PRR pathway deals with DNA damage in an error-free manner.
While the combination of structural observations and mutagenesis data clearly highlights the functional contribution of the C-terminal portion of the Bridge Helix towards controlling the rate of the NAC, the role of the N-terminal portion of the Bridge Helix has thus far remained enigmatic.
For any given protein, the totality of the available mutagenesis data is related to the number of laboratories devoted to its function, and the diversity and complexity of standard and novel tools used to study the properties of wild-type and mutant forms of the protein.
Results of mutagenesis data are explained in context with the present three-dimensional structure.
In contrast to the site-directed mutagenesis data a major role of hPR Cys891 in progesterone recognition could not be confirmed.
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