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Use of multiple unique probes per transcript in general increases the confidence of microarray result.
For those genes represented by multiple unique probes, the probe with the highest average intensity was selected.
$$\end{document} Affymetrix microarrays, however, measure alleles A and B numerous times using multiple unique probes (oligos) scattered across the microarray's surface.
Tiling arrays also optimize the detection of viral genes by using multiple unique probes for one transcript, an advantage over low-density spotting arrays or individual primer/probe sets used for PCR-based detection.
The dataset was generated by additionally averaging the signal intensity of the multiple unique probes for each gene based on GeneSymbol as provided by Agilent in the annotation file.
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Based on the above-mentioned method for probe selection, many genes are represented by multiple but unique probes derived from different mammalian species.
It contained multiple (3 8) replicates of 3,098 unique probes and multiple replicates (8 80) of 56 control probes.
A custom designed miRNA microarray that incorporated multiple replicates of 3,098 unique probes for sheep and other mammalian miRNA was used.
The ability to probe multiple unique plasma metabolites simultaneously with complementary analytical techniques holds much promise for revealing altered metabolic pathways with disease, assessing diabetic complications, and monitoring the efficacy of therapeutic interventions.
After elimination of 146 transcripts with multiple hits on the 93-11 genome, 510,458 unique probes of 45,035 transcripts survived uniquely targeting both the Nipponbare and 93-11 genomes.
This is consistent with the notion that some genes are conserved across multiple regions that are interrogated by unique probes on the microarray, resulting in significantly more probes per probeset than random expectations.
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