These five classes are: Reference Sequence (the sequence found in the GRCh37/hg19 human reference assembly), Class 1, Class 2 (each contains multiple unique sequence differences compared to the Reference Sequence), Exchange 1 (contains the Exchange 1 sequence replacement polymorphism) and Exchange 2 (contains the Exchange 2 sequence replacement polymorphism).
However, by demanding that proteins are identified with multiple high quality MS/MS spectra (with FPR <1%) and multiple unique peptide sequences in multiple independent preparations, many of these contaminations can be eliminated.
For K12, multiple unique peptide sequences are detected within upstream repeats and may correspond to kaposin isoforms B or C (unannotated [ 16, 17]); all detected K12 peptide sequences are in the same frame.
With the exception of the MD_3 gene, which was identified in a single genomic clone, all of the tandem MS4A genes were identified in multiple unique genomic sequence clones suggesting these are not the product of artificial fusion events.
Multiple neuropeptides of unique sequences are secreted and utilized for coordinate regulation of physiological functions.
Multiple alignments of unique sequences from each sample were carried out using T-Coffee [ 34- 36].
Both putative clades are supported by multiple unique and identical sequence motifs in conserved alignment regions of ITS1, as shown in Fig. 4. X and Z are illustrative of the dominance of Australasian-derived sequences (12 ITS-types) in the set of 21 ITS-types recovered from only one biogeographical region (= 57%).
MOL-PCR was first described by Deshpande et al. [ 2] as a powerful tool for detection of microbial pathogens allowing to combine analysis of multiple types of markers like unique sequences, indels, repeats, or single nucleotide polymorphisms (SNPs) in a single multiplex reaction.
It was shown that 72.7% of the 33-mer sequences were just single read, 24.4% of those had multiple reads, and 2.9% of unique sequences were low quality sequences containing at least one unknown nucleotide 'N'.
ESTs that did not form contigs (singletons) and the contigs resultant of assemblage of multiple sequences were referred to as unique sequences.
