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In a somewhat analogous manner, recent evidence shows that multiple unique miRNAs can be generated and co-regulated from a single primary miRNA (pri-miRNA) transcript [ 49, 50].
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The observation that multiple unique small-RNA reads could be generated from the same miRNA precursor has already been investigated by other authors [ 34, 55, 65, 66].
Analyzing 711 unique miRNAs, we have identified several interesting miRNAs, including miR-1, -133, and -208, that have been previously reported to be involved in cardiac development and disease and that show surprising patterns of expression across our samples.
To the listener, it sounds like multiple, unique perspectives.
3,067 unique miRNA-mRNA pairs (involving 37 unique miRNAs) were identified using miRBase whereas 330 pairs (7 unique miRNAs) were identified using the TargetScan database.
Specifically, 180,557,942 reads produced 13,324 unique miRNAs representing 224 conserved miRNA families that have been identified in other plants species.
Final data included 15,929 unique genes and 848 unique miRNAs.
This resulted in 41 miRNA-protein relationships, representing 8 unique proteins and 26 unique miRNAs.
Similarly, NSCLC and SCLC specific miRNA-TF-miRNA or TF-miRNA-TF or miRNA-miRNA interaction maps were created using targets of NSCLC and SCLC unique miRNAs.
In total, 1372 unique miRNAs were detected across 188 libraries.
The biological role of unique miRNAs was also discussed.
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