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Overall, in Ghsr deficient mice, UAG modulated by greater than 2-fold the signal from 374 Probe Set IDs in WAT, 131 in muscle and 151 in liver (data corrected using the Benjamini-Hochburg multiple testing method, with effects considered significant at p<0.05).
An analysis of variance (ANOVA) was performed to determine differential expression levels between conditions and time points using the Bonferroni multiple testing method (p < 0.05).
The numbers of significant GO terms were large; therefore multiple testing method was performed in order to control the rate of errors.
FDRmax values for each contrast were calculated using the method of Benjamini and Hochberg [ 53], the default multiple testing method in Limma.
Multiple comparison correction by controlling the FDR, which provides a useful alternative to traditional multiple testing method, further corroborated that preoperative high ESR levels, but not ALP, were a significant predictor for CSS PFDRR adjusted <0.01).
For homogeneity, differentially expressed genes were identified by first filtering on a fold change ≥ 2 and then performing statistical tests using the Benjamini-Hochberg multiple testing method with a false discovery rate set at 0.05.
Similar(50)
Multiple testing methods normally used in microarray analyses to correct for false positives include FDR (false discovery rate) and Q-value [51] [53].
All patient samples were tested for HPV infection using multiple testing methods.
Responses different from controls were assessed for statistical significance by multiple testing methods (global error rate α = 5%, two-sided).
Significance in the multivariable model will take into account the number of variables tested using multiple testing methods such as the Bonferroni Correction.
In addition, we illustrated the use of appropriate multiple testing methods for microarrays in monitoring differential gene expression between different biological states.
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