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The CSR model was created by modified multiple platform method as stated earlier [ 19].
We employed the modified multiple platform method to create the REM-CSR rat model.
The modified multiple platform method used to create the CSR model had been repeatedly tested by previous researches [ 1].
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To compensate for the different capabilities of each technique/method, their bias toward specific classes of metabolites, and the wide physicochemical diversity of the metabolome in a biological matrix, multiple platforms and methods are required to expand metabolite coverage.
Using multiple fragmented programs that each lack some necessary features can increase staff burden and result in the needless generation of overlapping data structures (i.e., using multiple platforms or methods to collect the same data in a single treatment setting).
Indeed, this study suggests that comprehensive metabolite profiling of human serum requires multiple platforms and multiple methods as no single method can offer (nor likely will offer) complete metabolite coverage.
The advantage of the methods applied in this study is that multiple platform analyses could improve the reliability of single measurements.
This led us to test the orbital platform method.
To merge the microarray datasets measured with multiple platform chips, we selected genes from all platforms based on the NIH Entrez Gene ID and used the Cross-Platform Normalization (XPN) method of Shabalin et al. [ 40] implemented in the R package: "CONOR" [ 41].
Although we chose an amplicon-based method on Ion Torrent, the samples could also be run on multiple platforms or used in a capture-based method [ 29].
MinION-called variants and haplotypes were validated against statistically phased genotypes from multiple platforms including Complete Genomics and Sequenom MassARRAY (see Methods).
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