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Exact(7)
After 5 min, 2 mm of distal mouse tail was removed by scalpel.
The DNA extracted from mouse tail was analyzed by PCR using the following primers.
One centimeter of mouse tail was rinsed in 70% ethanol three times and consequently with PBS to remove excess alcohol.
A peripheral blood sample collected from the mouse tail was dropped onto glass slides precoated with acridine orange (1 mg/mL) and covered with a coverslip.
Genomic DNA extracted from mouse tail was digested with AflII, subjected to electrophoresis on a 1 % agarose gel and transferred to a Nylon membrane (Roche), positively charged.
The base of the mouse tail was aligned with and taped to the bottom of a centrally located, vertical, flat, metal bar that was connected to a force transducer which recorded the time the mouse spent in an immobile state.
Similar(53)
In those experiments adhesion of MDA-MB-231 human breast cancer cells to bovine cortical bone slices and sections of developing trabecular bone from neonatal mouse tail were assessed.
Genomic DNA from tips of mouse tails was isolated and genotyped using the REDExtract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich), according to manufacturer's instructions.
The distance between the heat source and the mouse tails was 1.5 cm and the application site of the heat on the tail was maintained within 2 cm.
Type I collagen isolated from mouse tails was dissolved in 0.013 mol/L HCl to achieve a concentration of 5 mg/mL.
DNA extracted from 2lox mouse tails was treated with VspI (New England Biolabs Japan, Tokyo, Japan) and hybridized with DIG-labelled DNA probes synthesized with the DIG PCR Synthesis Kit (Roche Diagnostics Japan, Tokyo, Japan).
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