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Mouse sera was obtained from CBA/J mice.

The antibody titer in the selected mouse sera was more than 1/50,000.

The toxicokinetics of ricin in mouse sera was shown to be biphasic (Fig 3).

The stability of the conjugates in buffer and in mouse sera was measured by HPLC at room temperature (RT) over a period of 1 to 120 hrs.

Serum specific peptides were selected by two rounds of iterative affinity selection and specificity of phages for different mouse sera was assessed by dot blot (according to the method described in [40]).

Virus neutralization activity of mouse sera was tested by plaque reduction assay.

Importantly, the immunized mouse sera were able to neutralize HIV strains including those belonging to clades B and C.

The overall recoveries of LE300 and its N-methyl metabolite from mouse sera were in the range 97.9 101.5% with %RSD ranging from 0.98% to 3.63%, which were in line with ICH guidelines.

Deer mouse sera were heat inactivated in a 55°C water bath for 30 minutes.

For IgG subtype analysis, mouse sera were tested at a single 1∶ 1000 dilution.

To define the neutralizing antibody responses, mouse sera were pooled by groups and pseudotype neutralization, and microneutralization assays were performed.