First, the spatiotemporal expression of Cep290 in the mouse brain was determined.
The mouse brain was initially imaged using hemoglobin as the endogenous optical contrast.
Next, infusion of albumin into a naïve mouse brain was used to test if albumin exposure is sufficient to induce the PNN degradation.
The mouse brain was insonified with a succession of ultrasound plane waves and the backscattered echoes were recorded and beamformed to produce an echographic image for each transmission.
Each mouse brain was embedded in 3% agarose and 50 µm-thick, free-floating coronal sections were cut in the Leica VT1000S vibrating microtome.
"The total number of human brain cells in the mouse brain was less than one in a thousand.
We found that clearance of C. albicans at days 4 and 7 from mouse brain was strongly impaired by the lack of APP, but conversely was markedly enhanced in 5xFAD mice (Fig. 5a).
The distribution of [18F]FTC-146 in mouse brain was further evaluated by high-resolution ex vivo autoradiography.
The level of tracer accumulation in all regions of S1R-KO mouse brain was comparable with that observed in muscle.
Furthermore, microarray analyses showed that the expression of OS-related genes in the mouse brain was also changed.
The mouse brain was segmented into 33 structures, including the hippocampus, amygdala, hypothalamus, thalamus, as well as fiber tracts and ventricles.
