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Isolation of the mouse muscle tissue was described before [30].
We performed a Ca2+ titration experiment for the binding interaction of alpha-tubulin and dysferlin in mouse muscle tissue.
Analysis of the co-immunoprecipitates revealed that Ca2+ was not required for alpha-tubulin binding by native dysferlin in mouse muscle tissue (Fig. 5B).
Here we first characterize the phenotype of EC from skeletal muscles by staining mouse muscle tissue for Sca-1, CD31 and CD34.
Altogether, these results show that some genes that are known to play an important role in embryogenesis are also detectably expressed in adult mouse muscle tissue.
This set of data, therefore, provides a benchmark of expression levels of different genes within mouse muscle tissue, something that was not possible to obtain reliably with microarrays because of variation in sensitivity of hybridization among the probes [ 10].
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The β-gal mRNA was present in all the mouse muscle tissues since the β-gal gene is endogenous.
Patient and mouse muscle tissues were fixed in 2.5% glutaraldehyde, processed as previously described (Napoli et al., 2011) and post-fixed in 2% osmium tetroxide for 1 h.
Stocks of mouse-adapted wild-type and drug-resistant virus were made as follows: Using viral stocks obtained from mouse muscle tissues harvested 4 days post infection, plaque assays were performed by adding overlays containing V-073, guanidine or no drug.
As shown in Figure 3 using RT-PCR, nsp4 RNA expression was detected only in mouse muscle tissues injected with the pSIN-β, not in the ones injected with the pCMV-β or the pSIN-β-Δnsp, demonstrating that only the pSIN-β was capable of expressing the nsp genes in vivo.
In this study, the biosafety of G and GO in vitro was evaluated by co-culture with bone marrow mesenchymal stem cells (BMSCs) and biosafety in vivo was observed by implanting materials into mice muscle tissue.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com