Sentence examples for mouse muscle cDNA from inspiring English sources

PCR resulted in two differently sized Rnf14 amplicons from mouse muscle cDNA.

Mouse muscle cDNA was used as template for a PCR designed to amplify the full length mRNA encoding the Rnf14 gene.

The Rinl 5′-end was amplified from mouse muscle cDNA using the following primers: 5′-CTGAGCAGCCTTGAGTCTTGTCTT-3′ (located upstream of the predicted start codon) and 5′-CCTGGCCAGAGCACGGATGT-3′.

To identify proteins that bind to MuSK, a mouse muscle cDNA library was screened with the MuSK cytoplasmic domain using the yeast two-hybrid system.

The MuSK bait was screened against a mouse muscle cDNA library (a gift from Dr. Chamberlain, University of Michigan Medical School, Ann Arbor) cloned into the pACT2 prey vector [31].

The coding sequences of the AnkR constructs, including AnkR_repeats (residues 42 853), and the full length AnkR C-terminal domain (residues 1529 1907), were PCR amplified from a mouse muscle cDNA library.

Full-length mouse p50 was amplified by PCR from mouse skeletal muscle cDNA using the primers: forward- EcoRI (5′-CGGAATTCCGGCATGGCAGACGATGATCCCTAC-3′) and reverse- SacII (5′-ATCGCCGCGGCGCTAATGGGTGACCCCTGCGTT-3′), digested with EcoRI/ SacII, and inserted into a N-terminal FLAG-tag mammalian expression vector.

The yeast two-hybrid system (Y2H) was used to identify potential TRIM32-interacing proteins from a mouse skeletal muscle cDNA library.

The bait strain was created by transforming L40 with TRIM32-pHybLex/Zeo and used to screen a mouse skeletal muscle cDNA library (Invitrogen), as previously described (30).

Plectin has been cloned from a rat glioma C6 cell cDNA library (Wiche et al. 1991), a human placenta cDNA library (Liu et al. 1996), and a mouse skeletal muscle cDNA library (Fuchs et al. 1999).

In order to obtain the amplification efficiencies for each gene, standard curves were elaborated by using 21 days pi Querétaro-infected-mouse skeletal muscle cDNA serial dilutions (range 1 100 ng).