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SAA3 expression in the mouse colon was primarily localized to the colonic epithelium.

Characterisation of the mouse small intestinal stem cell transcriptome identified a number of highly enriched genes including OLFM4, 30 which, although not expressed in the mouse colon, was also enriched in human colonic stem cells.

The uptake of nanoparticles in the inflamed mouse colon was assessed in a horizontal diffusion chamber.

The effect of different doses of DMH on mouse colon was also investigated to obtain a minimum dose of DMH which can induce visible lesions in mice colons at a high incidence.

The total number of MT-immunopositive crypts per mouse colon was determined by the sum of single immunopositives and all immunopositives within patches.

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In studies of female non-obese diabetic (NOD) mice, which have a high proclivity for T1D, it has been shown that when the male NOD mouse colon is colonized with female feces, the risk of T1D is identical to that of the female mice.

When compared to humans and pigs, the mouse colon is shorter and the caecum is larger, relative to colon size [33], [34].

In summary, we showed that SAA3 expression in the mouse colon is increased by the presence of the gut microbiota, and that this may be partially mediated through the TLR/MyD88/NF-κB signaling pathway.

Moreover, when excised segments of mouse colon were scratched after treatment with WGA, the copious mucus secretion normally observed from the cells thus wounded (Fig. 3c, green stain) was dramatically reduced (Fig. 3d), while FM 4-64 positive dead cells were strikingly increased in density along the injury site (Fig. 3d, red stain).

Thus, the Gpr119 mucosal responses in the mouse colon are not dependent on GLP-1.

After examination under a dissecting microscope, individual biopsies from standardised areas of the mouse colon were collected.

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