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The second most downregulated gene, interestingly, was SUB1B (Loneof09g11480), one of the three ERF factors within the Sub1 QTL (Xu et al., 2006) (Table 2, Fig. 2 and Additional file 1: Figure S1a).
Hypocretin was the most downregulated gene in human narcolepsy brains.
Ing2 was the most downregulated gene (Table S1), confirming the validity of the experimental system.
Leucine rich repeat neuronal 1 gene (LPRN1) was the most downregulated gene with a minus 2.46 fold change.
The fact that Mecp2 was as the most downregulated gene in KO mice confirmed that the experiments were reliable (Table 2).
The most downregulated gene was Membrane Metallo-Endopeptidase (MME), also known as common acute lymphocytic leukemia antigen, CALLA, CD10 or NEP, that is highly expressed in mature germinal center B-cells.
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Among the most downregulated genes by CENP-A silencing are SKP2, RAD51, and CCNG1.
Among the most downregulated genes were SKP2, RAD51, and CCNG1, whose function is related to cell-cycle progression [31] [33].
The five most downregulated genes of our microarray study (Gprasp1, Sc4mol, Calb1, Fabp7 and Itm2a) were also significantly more weakly expressed in all the brain tissues studied in the Mecp2 null mice (Table 3 and Figure S3).
ACTB and ACTBL3 belonged to the most downregulated genes.
Surprisingly, among the most downregulated genes we found several genes involved in digestion.
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