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To examine any morphological defects, we observed mature grains under a bright field microscope but did not found any differences between WT and mtd1 1 mutant pollen.
Alternatively, it is possible that bacterial factors interact with other non-immune-related developmental pathways to create the morphological defects we observe.
To avoid gross morphological defects, we tested different concentrations of DAPT (75 µM, 150 µM, 166 µM) on controls and nb/nbl morphants treated from 2-ss to 26 hpf, when primitive hematopoietic commitment and determination take place.
To obtain a clearer picture of the morphological defects, we used the sections to generate three-dimensional reconstructions of the inner ears of three rescued swr fish (two bmp2b-rescued swrta72 and one smad5-rescued swrtdc24) and two wild-type fish.
On the basis of the morphological defects we observe in a number of eggs these targets may include mRNAs involved in follicle cell migration or actin organisation during oogenesis, such as bullwinkle (bwk) or chickadee (chic).
Because staging of embryos depends on morphological criteria, and foscrb Y10A mutant embryos show morphological defects, we imaged control and mutant embryos always in parallel, and stages were classified according to elapsed time after egg collection, i.e., after equal developmental times (see Materials and methods for details about staging and imaging).
Similar(53)
Although the axonal MT cytoskeleton had no obvious morphological defects (data not shown), we observed abnormalities in the distribution of proteins and synaptic vesicles along the nerves.
To investigate whether the observed defects were specific for the mis-regulation of wnt1 expression following nipblb loss of function or were caused by morphological defects in hindbrain formation, we analyzed the expression of atoh1, a marker of hindbrain dorsal progenitors and ventricle opening.
We discovered morphological defects in eyes, wings and legs.
However, when analyzed by transmission electron microscopy, we observed morphological defects in the mitochondria of misc-1 animals.
In this study, we report morphological defects in neurons and neuromuscular defects in mutants of the C. elegans CAMSAP, ptrn-1.
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