Sentence examples for monitor protein loading from inspiring English sources

Cell-free extracts (60 µg protein) were loaded on 12% 1-D SDS PAGE (Invitrogen) and resolved proteins from several mini-gels were transferred to the same PVDF membrane (Millipore) so that one set of samples was used to monitor protein loading using β-Actin.

HSC70 was used to monitor protein loading.

The same blot was reprobed for β-actin to monitor protein loading on the blot.

Blots were stripped and reprobed with a β-actin antibody to monitor protein loading.

To monitor protein loading, actin levels were determined using mouse anti-actin mAb C4 (ICN Biomedicals, Aurora, OH).

Whole cell lysates were analysed by SDS-11% PAGE and immunoblot analysis as above, using anti-HA to detect T3S translocated ExoS effectors, anti-RalA to assess T3S translocated ExoS-ADPRT activity, and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (Millipore, Temecula CA) to monitor protein loading of whole cell lysates.

Mouse anti-GAPDH antibody was used to monitor equal protein loading.

Ponceau staining was used to monitor equal protein loading and transfer.

Ponceau staining of membranes was used to monitor protein transfer and loading.

Differences in protein loading were monitored by probing membranes with monoclonal anti-α-Tubulin antibody.

Protein loading was monitored using a mouse monoclonal antibody against β-actin (1 30 000, Sigma-Aldrich).