Sentence examples for monitor protein aggregation from inspiring English sources
As structural transition to intermolecular β-sheets occur regardless of the initial secondary structure composition of the native protein, these bands can be used to monitor protein aggregation in aqueous solution and solid state [ 33].
DTT-denatured bovine αLactalbumin (Sigma L6010) was used as a model substrate and light scattering at 360 nm was used to monitor protein aggregation in the presence and absence of WT- and mutant HSPB5 at 42°C.
Light scattering intensities (I) were integrated as correlograms (plots of a correlation coefficient, G = versus time, where τ = the sampling time of the correlator) and were used to monitor sample dispersity and protein aggregation.
As protein aggregates during denaturation (Zhao et al., 1998), we monitored the progress of protein aggregation in CuZn-SOD solution by using Rayleigh scattering.
In in vitro experiments, thioflavin T fluorescence can be monitored in real-time during protein aggregation to determine the kinetics of fibril formation.
The protein aggregation in the SOD solution was monitored by Rayleigh scattering by using the FluoroMax-4 Spectrofluorometer (Horiba scientific, Kyoto, Japan), as described in Liu et al. (2009).
We monitored incorporation of k6W-Ub into Ub-protein conjugates, deubiquitination, protein aggregation, cell proliferation, and differentiation, including lens-specific-crystallin expression, proper fiber formation, denucleation and lens clarity.
To investigate protein aggregation induced by elevated temperatures, we also monitored light scattering of the samples during its heating from 15 up to 80 °C with 1 °C/min rate.
Protein aggregation induced by modulating conditions in solution (e.g. thermal stress and agitation) was monitored over discrete time periods.
One thing I didn't mention in the article, due to space restrictions, is the relationship between protein aggregation and neurodegeneration.
(C) Quantitative analysis of protein aggregation in HEK 293T cells.
