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Taking advantage of this characteristic of 2D-PAGE gels enabled us to focus on highly expressed proteins, and to monitor protein expression that increased with increasing P removal performance.
Use of antibody arrays to monitor protein expression in exposed monolayers identified strong upregulation of phospho-keratin-8 (Ser73) as an early consequence of acrolein exposure.
While performing transient expression assays using agroinfiltration to monitor protein expression and localisation in N. tabacum leaves infected with the pathogen P. syringae pv.
The N terminal tag was necessary to monitor protein expression and to increase fragment size to facilitate analysis by SDS-PAGE.
SDS-polyacrylamide gel electrophoresis was used to monitor protein expression and purity.
The INPUT represents 2% of total lysate to monitor protein expression (top panel).
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In earlier reports, insulin receptor has been demonstrated in cultured porcine granulosa and theca cells by monitoring protein expression of downstream targets such as IRS-1, GLUT-4, and PPAR- γ [ 20, 25].
Furthermore, we have provided protocols and methods to monitor intracellular protein expression and trafficking as well as evaluation of the UPR, with essential controls.
Immunohistochemistry was used to monitor the protein expression of Cyr61, CTGF, and WISP-1 in the 60 pairs of lung tissues.
The culture was further incubated under shaking at 30°C for 24 hours, with 5 mL sub-sample collection at 0, 4, 8, 12 and 24 h to monitor the protein expression.
Recently monitoring the protein expression pattern in clinical specimens by proteomics technologies has offered great opportunities to discover potentially new biomarkers for cancer diagnosis.
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monitor protein loading
monitor protein conformation
monitor protein quality
monitor protein oxidation
monitor protein adsorption
monitor protein modification
monitor transgene expression
monitor protein folding
monitor protein stability
monitor protein tertiary
monitor protein aggregation
monitor cyclin expression
monitor surface expression
monitor protein distribution
monitor protein kinase
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