Sentence examples for monitor protein stability from inspiring English sources

We then will briefly address immunogenicity concerns and outline current best practices for using state-of-the-art analytical assays to monitor protein stability for both conventional and novel therapeutic protein dosage forms.

This work demonstrates that His-HDX-MS is complementary to amide HDX, and to other conventional methods such as fluorescence spectroscopy and CD spectroscopy for monitoring protein stability.

We then monitored protein stability at the times indicated in the Figure 4. Cyclin D1b exhibited a half-life similar to cyclin D1a in both cell lines (indicated under each immunoblot).

To determine if the remaining 37 compounds interact directly with E6, a fluorescent E6 thermal stability assay was employed to monitor protein unfolding in the presence of a given inhibitor.

To rule out an impact of H2O2 on translation or mRNA stability, we monitored MBD4 protein stability.

A triple strep tag sequence was introduced at the 5′-end of the gene constructs to allow monitoring of protein stability by Western blot analysis.

Protein stability was monitored after the protein synthesis had been inhibited by the addition of 25 µg/ml chloramphenicol to bacterial cultures grown to OD600nm at 2.0 in LB medium at 37°C.

A FLAG-tagged plasmid of NRF2 was thus transfected into cells along with either SAP130 or empty vector and protein stability was monitored over time.

Overall, we demonstrate the excellent potential of "temperature profiling" by RP-HPLC to monitor differences in oligomerization state and protein stability.

Consistently, the protein stability of VHL where monitored by ubiquitin or SUMO modification and showed reduced stability of VHL-UbΔGG compared to VHL-SUMO1ΔC4 and may explain why VHL was more stable once lysine 171 was mutated (Figure 4B).

While steady-state methods provide valuable insights into intracellular behaviors of IDPs, the ability to quickly manipulate the cellular environment and monitor relaxation kinetics provides important additional information on protein stability and the conformations accessible to IDPs and folded proteins.