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Using data from the crystal structure of an initial hit compound, we have developed a fluorescence polarization assay to monitor binding of compounds to the ATP-binding site of Hsp90.
With refractive index sensing, the nanoCA could monitor binding of TNT to the peptide, while the peptide was immobilized on nanoCA by Au S covalent linkage.
This approach allows one to monitor binding of transcription factors to their recognition elements by measuring the frequency of binding-site dependent DNA cleavage, after in vivo crosslinking, by micrococcal nuclease that was linked to the transcription factor [26].
Differential bleaching was used as a parameter to monitor binding of MBP-NES proteins to CRM1.
Next, a fluorescence polarization (FP) assay was developed to monitor binding of inhibitors to DOT1L using the fluorescent probe 2.
Although the change in the far-UV CD signal is small, it can be used to monitor binding of a metal ion to F2 TnC.
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(G ) ChIP analysis monitoring binding of cJUN to the USP28 promoter or an irrelevant negative control (NC) DNA region.
(L ) ChIP analysis monitoring binding of CDX1 to the PRKD1 promoter or an irrelevant DNA region (NC) DNA.
The system automatically monitors binding of a fluorescent dye to double-strand DNA by real-time detection of the fluorescence emitted during each cycle of PCR amplification.
(C ) EMSA titrations monitoring binding of yPrp3CTF variants and yPrp3DUF1115 (proteins indicated at the left of the gels) to yU4/U6stem II+13nt (scheme on the top).
ChIP analysis monitoring binding of ZNF304, KAP1, SETDB1, and DNMT1 at the promoters of p14 ARF, p15 INK4B, and p16 inK4A in HCT116 (left) and HCT15 (right) cells.
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