Sentence examples for monitored binding of from inspiring English sources

To assess the advantage of IgM + J-chain constructs by flow cytometry, we monitored binding of mouse PD-L1 molecule to CD3+ primary mouse T cells which express its ligand PD-1 when activated.

In addition we also monitored binding of the three PcG protein complexes PhoRC, PRC1 and PRC2 by performing X-ChIP reactions with antibodies against the PhoRC component Pho, the PRC1 component Ph and the PRC2 component Su z 12.

We monitored binding of ISWI to this substrate using the same native gel analysis and fluorescence anisotropy assay; the resulting data suggest that ISWI binds to this substrate with the same affinity and stoichiometry as the F10N5F substrate.

To corroborate our cytological method, we isolated total nucleic acids from wild-type, sin3Δ (a representative RNA biogenesis mutant), and sin3Δ rad51Δ cells, transferred them to a solid matrix and monitored binding of S9.6.

Arp2/3-SNAP was tethered to the slide surface via a bi-functional SNAP substrate that incorporated both a Dy649 dye and a biotin-terminated PEG chain; we monitored binding of fluorescently labeled diVCA and actin filaments from solution.

Using data from the crystal structure of an initial hit compound, we have developed a fluorescence polarization assay to monitor binding of compounds to the ATP-binding site of Hsp90.

With refractive index sensing, the nanoCA could monitor binding of TNT to the peptide, while the peptide was immobilized on nanoCA by Au S covalent linkage.

This approach allows one to monitor binding of transcription factors to their recognition elements by measuring the frequency of binding-site dependent DNA cleavage, after in vivo crosslinking, by micrococcal nuclease that was linked to the transcription factor [26].

Differential bleaching was used as a parameter to monitor binding of MBP-NES proteins to CRM1.

Next, a fluorescence polarization (FP) assay was developed to monitor binding of inhibitors to DOT1L using the fluorescent probe 2.

The system automatically monitors binding of a fluorescent dye to double-strand DNA by real-time detection of the fluorescence emitted during each cycle of PCR amplification.